Journal: Endocrinology

Article Title: Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex.

doi: 10.1210/en.2004-0179

Figure Lengend Snippet: FIG. 5. Effect of Dex treatment on adrenal cortex vasculature and VEGF expression. Sections of adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14) were immunostained for CD31 (A) or VEGF (B). The dotted lines indicate the border between adrenal cortex and medulla. Immunoreactivity is indicated by brown staining. Data from one representative experiment are shown. Similar results were obtained in three independent experiments.

Article Snippet: Slides were then incubated for 1 h with 0.5 g/ml rabbit polyclonal antihuman VEGF antiserum (A20; Santa Cruz Biotechnology, Santa Cruz, CA), which recognizes all VEGF isoforms or with a rat anti-BrdU at a dilution of 1/75 (clone BU1-75; Harlan, Indianapolis, IN).

Techniques: Expressing, Staining

Journal: Endocrinology

Article Title: Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex.

doi: 10.1210/en.2004-0179

Figure Lengend Snippet: FIG. 6. Effects of Dex treatment on the expression of endocrine and endothelial cell markers. A, RT-PCR analysis of the expression of VEGF-A isoforms (the two bands correspond to specific amplification products of the VEGF120 and VEGF164 isoforms), VEGF-Rs (VEGF-R1/flt-1, VEGF-R2/flk-1, and neuropilin-1), endocrine cell markers (ACTH receptor/MC2-R, HDL receptor/SR-B1), and endothelial cell markers (PECAM and VE-cadherin) in the adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14). RT-PCR amplification of HPRT mRNA was used as an internal standard for the normalization of the samples. The results from one representative experiment are shown. Similar results were obtained in three independent experiments. B and C, Quantitative determination of adrenal VEGF-A and VEGF-Rs mRNA levels by real-time RT-PCR was performed as described in Materials and Methods. The ratio of level of expression of the gene of interest to that of GAPDH was normalized to 1 in control adrenals. The relative quantities of the following mRNAs were plotted as a function of time of Dex treatment: VEGF-A (B); VEGF-R1 (C, solid line), VEGF-R2 (C, continuous dotted line), and neuropilin-1 (C, discontinuous dotted line). Each value represents the mean SEM from 10 to 15 mouse adrenals collected in three independent experiments.

Article Snippet: Slides were then incubated for 1 h with 0.5 g/ml rabbit polyclonal antihuman VEGF antiserum (A20; Santa Cruz Biotechnology, Santa Cruz, CA), which recognizes all VEGF isoforms or with a rat anti-BrdU at a dilution of 1/75 (clone BU1-75; Harlan, Indianapolis, IN).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Quantitative RT-PCR, Control